Experimental primary ocular canine herpesvirus-1 infection in adult dogs | American Journal of Veterinary Research
After streptavidin-alkaline phosphatase conjugate (UMap anti-Rb AP, Ventana Medical Systems) incubation for 24 min at 37°C, the signal was detected automatically using the BlueMap NBT/BCIP substrate kit (Ventana Medical Systems) for 44 min at 37°C. Briefly, Madin-Darby canine kidney (MDCK) cells (ATCC, Manassas, VA) were grown in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, St Louis, MO) supplemented with 10 % fetal bovine serum (FBS) (Sigma-Aldrich), penicillin (200 U/ml), and streptomycin (200 mg/ml). The nasal turbinates were dark red to black. After streptavidin-alkaline phosphatase conjugate (UMap anti-Rb AP, Ventana Medical Systems) incubation for 24 min at 37°C, the signal was detected automatically using the BlueMap NBT/BCIP substrate kit (Ventana Medical Systems) for 44 min at 37°C. For nucleotide sequencing, viral DNA (a portion of the DNA polymerase gene) was amplified from isolate 08/005 by the PCR with herpesviral consensus primers TGV (5′-TGTAACTCGGTGTAYGGNTTYACNGGNGT-3′) and IYG (5′-CACAGAGTCCGTRTCNCCRTADAT-3′), as described previously (33). and Huiskonen, J.T. (13), was used for amplification of the nucleoprotein gene of canine distemper virus.
However, in the encoded protein (92 aa long), while the aa identity to strain MPV/J3666 was again high (97.8%), MPV/15 showed a lower relatedness with 92.4% of aa identity. We estimated pups born per pack based on high counts observed between May-June. Twenty samples, including 10 that were positive and 10 that were negative by EBV VCA IFA, were tested in a CHV-1 neutralization assay, but none was positive, indicating that positivity did not result from cross-reactivity with CHV-1. The present case report corroborates previous observations that CnMV can act as an infectious agent of neonatal mortality in dogs.7 Both CnMV and Canid herpesvirus 1 may cause reproductive failure and puppy mortality, but the latter virus has been isolated with more frequency from outbreaks of neonatal death.7,11 The paucity of reports of the CnMV-associated disease may reflect failure to submit dead neonatal puppies to laboratory diagnosis, especially when death involves single members of a litter. However, when recent CnPnVs were included, the aa identities varied from 89.6% to 95.9%. Samples were analyzed for the presence of herpesvirus, poxvirus and calicivirus. Oral antibiotics cure the infection.
Dla odmiany pierwotne zakażenia u suk ciężarnych w większości przypadków prowadzą do ronień, resorpcji lub mumifikacji zarodków/płodów, a infekcje u dorosłych zwierząt i starszych szczeniąt mogą manifestować się zmianami w obrębie błon śluzowych czy oczu lub przebiegać, jako tzw. Cats and dogs are the commonest domestic animals and pets worldwide. Using a degenerate herpesvirus polymerase gene PCR assay, positive results were obtained in two of three B-cell lymphoma samples. Erythematous skin lesions involving the entire body and footpads (a, b, c). Fluorescent antibody staining for leptospiraa was also negative. RT-PCR with consensus primers amplifying a 155-bp fragment in the L gene of morbilliviruses was positive in samples (53 urine, four rectal swabs, and one blood sample) from 56 (12.3%) of 457 stray cats captured from various locations in Hong Kong over a 2-y period (March 2009 to February 2011), with two stray cats positive on both the urine and rectal swabs. These results suggested that EBV may have been transmitted from human to dog.
Ulcerative and non-ulcerative keratitis were less prevalent and of variable clinical appearance. EnglishActive immunisation of bitches to prevent mortality, clinical signs and lesions in puppies resulting from canine herpes virus infections acquired in the first few days of life. Please submit dry, plastic-stemmed swabs, without transport media, in an RTT or an empty, sterile tube; keep refrigerated. Diagnosen blev stillet på baggrund af de patologiske fund. In shelter #2, 9% (3/35) of the samples were positive for Canine parainfluenza virus (CPIV), and 6% (2/35) were positive for Canid herpesvirus 1 (CaHV-1). The characteristic gross lesions consist of disseminated focal necrosis and hemorrhages, primarily in the lungs. Carcasses are provided to these organisations mainly as a result of land and estate management, or sometimes as RTAs or natural causes of death.
Similar to other animal models of recurrent HSV-1 ocular infection, the behavior of latent CHV-1 in dogs may differ from HSV-1 in humans; however, results of the present study suggest administration of topical ocular prednisolone at the evaluated drug concentration, dosing frequency, and treatment duration is not likely to result in detectable reactivation of latent CHV-1 in experimentally infected dogs. Swoją współczesną nazwę zawdzięczają jednemu z najwybitniejszych prekursorów współczesnej medycyny – Hipokratesowi, który już w odległej starożytności opisywał zmiany skórne o charakterze pęcherzykowatym nękające ludzkość. Complete: Journals that are no longer published or that have been combined with another title. The present results serve as a warning to canine clinics that, under the specific set of circumstances described, such serious CHV outbreaks may be expected wherever canine ITB occurs. The cases in the current report document lesions observed in association with CaHV-1 in 4 cases of fatal canine herpesvirus pneumonia in adult dogs. Molecular and serological analyses revealed that the causative CHV isolates were from a single source but that none was a peculiar strain when the strains were compared with previous CHV strains.